An autoclave is more or less similar to a pressure cooker, except that the autoclave moves more vigilantly. This is explained by concerns that the tools do not contain any microorganisms, so there is little room for error when sterilizing. The autoclave has a main compartment where the equipment to be sterilized is placed. After this, the chamber was closed and the device turned on to direct the dense vapor cloud into the compartment. During this process, the temperature and pressure in the chamber begin to increase with the aim of reaching and maintaining a temperature of about 120 degrees Celsius.
But would the steam be pushed into the chamber cleaning device like this? What happens to dead microbes? An autoclave is more advanced because it contains pipes and valves that allow the exchange of air inside. These pipes allow pure steam to enter the chamber and push contaminated air out of the chamber. A typical sterilization process requires about 15 minutes of steam at 120 degrees Celsius, but please note that this is not standard as it depends on the type of equipment being sterilized. Some combinations are done with additional hot steam, allowing the material to be sterilized in a shorter period of time. Extremely high temperatures destroy the interior of the microbes, thus eliminating any chance of survival. After the sterilization period, the steam is removed and the pressure is gradually reduced. Effective sterilization depends on the use of saturated steam to displace contaminated air, so exposure to this pure steam ensures effective sterilization.
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